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Older GLP-1 medications like exenatide (Byetta) required twice-daily dosing, but this is no longer the standard

To obtain a GLP1-RG s complex with good homogeneity and stability, we used the NanoBiT tethering strategy, in which the C-terminus of rat G1 was linked to HiBiT subunit with a 15-amino acid polypeptide (GSSGGGGSGGGGSSG) linker and the C-terminus of GLP-1R was directly attached to LgBiT subunit followed by a TEV protease cleavage site and a double MBP tag (Supplementary Fig

That made direct comparisons imprecise at best and misleading at worst

HardcastleSJHancoxJHattarAMaxwell-SmithCThgersen-NtoumaniC

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