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In bacteria, the evolution of protein paralogs that perform identical functions in cells, but with distinct inorganic cofactor requirements or none at all, is a particularly dramatic and sometimes overlooked example of metabolic and evolutionary adaptation to bioavailable metals

BZHU: proteins 0 g, fats 0 g, carbohydrates 0 g

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2) Transfer an appropriate-sized slurry to the column and allow the agarose to settle down and the storage buffer to drain from the column