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BDNF cloning Rat total DNA was extracted from a C6 rat glioma cell line using the GenElute mammalian genomic DNA purification kit (Sigma-Aldrich, St Louis, MO, USA), and the coding sequence of the BDNF gene was subsequently amplified by PCR using an Expand High Fidelity DNA polymerase (Roche Diagnostics, Penzberg, Germany)

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Similar to glutathione, enzymatic antioxidant activity in plants subjected to increasing water stress regimes was enhanced, with the exception of SOD in S nemorosa