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10.1093/HUMUPD/DMAD006 43 VassenaR.BouS.Gonzlez-RocaE.AranB.AuerH.VeigaA.et al (2011)

The samples were centrifuged at 15,000 rpm for 2 min at 4 C using an Eppendorf 5424R centrifuge (Eppendorf SE, Hamburg, Germany), and the resulting supernatant was subjected to HPLC-MS/MS analysis