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Our findings indicate a non-canonical role for GSH in supporting tumours by acting as a reservoir of amino acids

New insights into the molecular mechanisms underlying effects of estrogen on cholesterol gallstone formation

2.5 LCMS/MS untargeted metabolomics sequencing 2.5.1 Metabolite extraction Weigh 20 mg of each sample, add 200 L of pre-cooled 80% methanol water with 3 steel beads, homogenize and crush in a tissue crusher, then add 800 L of pre-cooled 80% methanol water, vortex mixing, sonicate for 2 h in an ice bath, and then let stand overnight at 20C

While this method is convenient, it may have limited efficacy due to the digestive breakdown of the peptide before it can be fully absorbed into the bloodstream

Bailey, Michelle