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To demonstrate whether a specific cytokine plays a role in angiogenesis after hindlimb ischemia, we measured mice serum cytokine levels using Luminex technology in four groups: WT sham ( n = 3), WT HLI ( n = 4), KO sham ( n = 3), and KO HLI ( n = 4) to compare cytokine releasing levels between WT mice and GSDMD -/- mice after HLI (Fig

For each gene, this process was repeated in approximately 5 tissue sections from 3 different mice, and the data were averaged as density of signals ( S.E.M)

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Peshock RM

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