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E3 is produced primarily from 16-hydroxy-dehydroepiandrosterone (DHEA) sulfate, 17-estradiol or E2 is formed from aromatization of testosterone, whereas E1 is derived from aromatization of androstenedione (Yaghjyan and Colditz, 2011)

Image credit: However, a small study conducted healthy Thai male volunteers has found out that coffee enema does not raise the level of glutathione S-transferase in the volunteers blood

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While morning intake may offer a slight edge for absorption on an empty stomach, consistency over weeks and months is far more important than the exact hour you take it