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Specifically, in the leaky gut state, gut microbiota dysbiosis occurs, the expression of intestinal epithelial junction proteins (e.g., tight junction proteins) decreases, and intestinal permeability increases, forming a leaky gut (165)

Missing data were assumed to be missing at random, and multiple imputation was performed by multivariate imputation using chained equations (38, 39)

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Through the VEGFR2-Akt-eNOS pathway, it promotes angiogenesis (new blood vessels), ensuring that the metabolically active fibroblasts stimulated by GHK-Cu receive the oxygen and nutrients needed for protein synthesis

Alongside this, they reduce glucagon output from alpha cells, which limits unnecessary glucose release from the liver and helps maintain steadier fasting and post-meal blood sugar levels throughout the day