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In addition to neuroimaging, peripheral blood testing including vascular endothelial growth factor, fibroblast growth factor, and -amyloid peptide 40 was performed, and advanced polychromatic flow cytometry was used to phenotype circulating mononuclear cells to assess angiogenic activity (Callahan et al

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Lafayette, CO) or On-Target plus siControl (Non-targeting pool, D-001810-10 Dharmacon) were transfected (50 nM for 5 h) using Lipofectamine 2000 (Invitrogen) (10 l per dish) according to the manufacturer's instructions

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