After adding 340 L 4 C cold mixture of methanol and acetonitrile (ACN) (25:75, v/v) and 50 L of a 2 M D 9 -trimethylamine-N-oxide (Cambridge Isotopes Laboratories, Tewksbury, MA, USA) solution in methanol, the protein was precipitated by vortex-mixing for 30 s followed by 10 min incubation
Label every aliquot with concentration, batch, date, and storage temperature
This work was supported by grants from European Genomic Institute for Diabetes (E.G.I.D., ANR-10-LABX-46), European Commission and Agence Nationale pour la Recherche (ANR-FXREn)
Development of a gene transfer-based treatment for radiation-induced salivary hypofunction
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