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ROS directly target both nuclear DNA and mitochondrial DNA (mtDNA), resulting in a dual damage effect

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10 mg / 2 mL = 5 mg/mL 250 mcg / 5,000 mcg per mL = 0.05 mL = 5 U-100 units 500 mcg / 5,000 mcg per mL = 0.1 mL = 10 U-100 units Loading versus maintenance draws Community protocols often use a smaller starting dose, then a maintenance dose once a target is reached