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RT-qPCR as well as western blot analysis demonstrated that OVX animal liver has higher level of pro-inflammatory cytokines, IL6 and TNF, both at the RNA and protein level in comparison to the control female rats, and the level of OVX-induced elevation of IL6 and TNF was reduced upon treatment with EB [Figures 2A (RT-qPCR analysis) and 2B (western blots, quantification in respective right panels, supplementary figure S1)]

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MOTS-c appears on the World Anti-Doping Agency (WADA) prohibited list under Section 4.4 as an AMPK activator