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Acknowledgements The authors thank Daniela Dietel and Heike Hofmann for excellent technical assistance

+ The effects of PT-141 typically begin within about an hour of injection, with increased desire lasting up to 12 hours or longer for some individuals

Hundred microliters of the prepared sample were then mixed with 12 L of the prepared magnetic bead-capture antibody complex and 120 L of the prepared HRP-conjugated antibody and incubated at room temperature for 30 min in 1.5 mL Eppendorf brown tube

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