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Duration of NFT-dependent increase in glutathione levels To determine the duration and the response of glutathione following withdrawal and re-addition of NFT, HepG2 cells were treated with NFT (20 M) for 48 h, followed by withdrawal of NFT for 24 h and a subsequent re-addition of NFT for an additional period of 24 h (Fig

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In parallel, mitochondrial fragmentation and stress signaling may alter the plasma membrane lipid microenvironment and reduce the efficiency of membrane protein insertion, thereby compromising OCTN2 anchoring and stability within membrane microdomains [114]