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Tissue was then cut into small pieces and shaken in dissociation buffer (PBS: 2% FBS, 1.0 mM DTT, 5 mM EDTA, 15 mM HEPES) for 30 min at 37 C to release epithelial cell fraction

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References Harrington, D.D., & E.H

Chronic Inflammation and Oxidative Stress Oxidative stress occurs when the body produces more reactive oxygen species (ROS) than it can neutralize