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RNA samples were treated with the TURBO DNA-free kit (Applied Biosystems, Foster City, CA), further purified through an RNease kit (Qiagen) and quantified with a NanoDrop-1000 (NanoDrop Technologies Inc., Wilmington, DE)

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Abdominal pain, nausea, vomiting, diarrhea, and vertigo have also been reported, and one trial found that the incidence of nausea, vomiting, and vertigo was dose-dependent (77)