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Before derivatization, 2 mL 0.2 M phosphate buffer was added

That's why absence of FDA approval here is about business case, not about lack of evidence

Research Square PrintNovember 4th, 2022

This overactivation causes an influx of calcium ions into neurons, which, in turn, activates calcium-dependent enzymes, such as calpain, nitric oxide synthase and phosphatase, resulting in the degradation of cytoskeletal proteins and damage to cell membranes