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10.1038/jcbfm.2013.184 109 Meja-ToiberJ.MontielT.MassieuL

Aug 1990;55(3):439-53

Figure 14: Diagram showing the contrasting functions of glucagon and insulin, credit: Carogonz11, Wikimedia

To confirm that the SAT1 flipper disruption cassettes had correctly replaced either the GDH2 or GDH3 gene after double crossover integrative transformation, the 5 and 3 junctions of disrupted alleles were PCR amplified