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Cells were pelleted by centrifugation (50 g for 5 minutes at 22 C) re-suspended in fresh media and the cell viability was determined by Trypan Blue exclusion

We found that the density of Ki67+ cells is significantly reduced in the subgranular zone of DG-NR1KO mice compared with control ( p = 0.023, t (11) = 2.641, n = 6 control and 7 DG-NR1KO mice, independent-sample t test, two-tailed

Because B12 is primarily found in animal products (meat, fish, dairy, eggs), people following vegetarian or vegan diets may be at higher risk of deficiency