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Ethical approval and consent to participate This investigation was conducted in full compliance with the 1964 Declaration of Helsinki and its later amendments, and the International Conference on Harmonisation Guidelines

FIGURE 4 Validation of RNA-seq results and the expression analysis of Wnt/-catenin signaling pathway-related genes To verify the results of RNA-seq, 13 differentially expressed genes, including 6 downregulated and 7 upregulated genes, were randomly selected for qRT-PCR assay