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a , UMAP of the distribution of cell clusters (down sampled 5 000 cells from 86 052 cells) from autoantibody injected (0.25, 0.5, 1, 2, 12, 33, 63 days) and control DRG samples
Ben-Shachar D
The acidified environment adds positive charge to the peptide, and that shared charge creates electrostatic repulsion between molecules
Cells were seeded at a density of 5000 cells per well in 200 l fresh culture medium (DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution) into 96-well flat-bottomed microtitre plates (Costar, Corning, USA) and incubated at 37 C in humidified 5% CO 2 using AutoFlow NU-5510 Direct Heat CO 2 Incubator (NuAire, USA) for 24 hours before treatment
