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Researchers should validate probe specificity using GSH-depleted controls, such as buthionine sulfoximine (BSO)-treated cells, to confirm that the signal reflects glutathione rather than other intracellular thiols

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12 Cellular swelling, karyolysis, karyorrhexis, vacuolization, inflammation and release of cellular contents (alanine aminotransferase, ALT) are key processes of hepatocyte necrosis and related death in humans, as shown by the biochemical evidence of severe elevations in aminotransferases, especially ALT

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