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Bulk RNA-seq data for skin of young mice (2 months old) and aged mice (18 months old) were obtained from GSE185087
The Annexin V-Alexa Fluor 488/propidium iodide (PI) dead cell apoptosis kit (Thermo Fisher Scientific V13241) was used to detect early and late apoptosis and necrosis as previously described [18]
If supportive measures such as supplementation, topical therapy, or sun protection are discontinued, pigmentation may gradually recur
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