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To build MD simulation systems, the complexes were embedded in a bilayer composed of 257 POPC lipids and solvated with 0.15 M NaCl in explicit TIP3P waters using CHARMM-GUI Membrane Builder v3.5 28

[33] [226] [227] In December 2017, the first study assessing the interaction between amphetamine and human carbonic anhydrase enzymes was published