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greater than 10mls) The Neck The land marks for injecting in the neck muscle are: The scapula (shoulder blade) at the base of the neck (behind the red line) The cervical spine (neck vertebrae) at the bottom of the neck (below the green line) The nuchal ligament at the top of the neck (above the white line) You should inject in the triangle approximately a hands width above the shoulder blade, about half way between the nuchal ligament and cervical vertebrae Hindquarters (Gluteals) The aim is to inject into the large muscle mass of the hindquarters Only inject in this area if the horse is of suitable temperament for you to do so safely Feel for the three bony prominences of the pelvis (the tuber coxae, tuber ischium and tuber sacrale) as illustrated by the green arrows Imagine a triangle using these three points and inject approximately in the centre of this triangle (as far from the bony prominences as possible) Technique Make sure area is clean Select the appropriate site for your injection Stand in an appropriate and safe position in case the horse reacts to the needle being inserted Quickly and decisively, insert the needle perpendicular to the skin

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Toyota et al

References 42 and 43 illustrated that the conditional elimination of bone marrow-derived, perivascular monocytes was sufficient to impair angiogenesis and delay tumour growth in transplant cancer models

Animals and genotyping The rat DA Gsta4 strain over-expressing Gsta4 under a CAG-promotor was purchased from Taconic Bioscience