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By contrast, for some indices, if there seemed to be no significant trend between groups with three repeats, we determined that there were no significant differences between groups and that the sample size would not increase

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Animals were treated with Dox and/or NMN, and 8 weeks later were stimulated with pregnant mares serum gonadotrophin (PMSG), with the number of germinal vesicle (GV) stage cumulus oocyte complexes (COCs) recovered from ovarian antral follicles used as a readout for oocyte yield (Fig