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And the research is growing

Polypodium leucotomos extract (PLE) has drawn attention as a potential treatment for melasma due to its antioxidant & photoprotective properties

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Following loading, the column was washed with 10 column volumes (CV) of Buffer A to remove nonspecifically bound proteins then followed by a gradient of Buffer B (50 mM Tris-Cl, 300 mM NaCl, 500 mM Imidazole 0.01% LMNG/0.001% CHS, pH 7.0) consisting of the following step sizes: 6% (10CV), 10% (2CV), 16% (2CV), and 24% (2CV)