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Aspiration (pulling back the plunger to check for blood) is an older technique generally considered unnecessary for subcutaneous injections with a short insulin needle

Despite its widespread use in cell culture studies, SLC7A11 inhibitor Erastin cannot be used for animal studies due to its poor metabolic stability and low solubility in vivo [13]

While that's a good thing, a recent study questions whether this reduces the risk of cardiovascular disease the main target of treatment

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doi: 10.1016/S0021-9258(18)89859-3 75 WhiteJWSaundersGF