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Following incubation, membranes were washed three times with TBST (5 min each) and incubated for 2 h at room temperature with alkaline phosphatase (AP)-conjugated secondary antibodies: goat anti-mouse (ab97020, Abcam 1:5000 dil.) or goat anti-rabbit (#ab6722, Abcam 1:3000 dil.), depending on the host species of the primary antibodies

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