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Nephron 131:167174 Kohagura K, Tana T, Higa A, Yamazato M, Ishida A, Nagahama K, Sakima A, Iseki K, Ohya Y (2016) Effects of xanthine oxidase inhibitors on renal function and blood pressure in hypertensive patients with hyperuricemia

Briefly, cells were suspended in sucrose-mannitol buffer (20 mM HEPES, pH 7.5, 2 mM EDTA, 70 mM sucrose, 220 mM mannitol, 5 mM NaF, protease inhibitor cocktail [Roche]) and homogenized using a Teflon homogenizer

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Our study identifies a protein that helps clear excess manganese, which is critical because too much of this metal can damage the brain. Working in collaboration with Yuhan Wang, assistant professor of nutritional sciences, and Aydemir's former graduate student, Jiaqi Zou, who is first author of the study, the team used advanced expansion microscopy to visualize how ZIP14 shifts within cells of the blood-brain barrier in response to excess manganese exposure