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The lack of awareness (60), paucity of diagnostic tools (60), heterogeneity between patients (26, 60, 529), disbelief from health care workers (60), unpredictable relapses, and multiplicity of symptoms have made it difficult to formulate a treatment for ME/CFS

This combined crude material was dissolved in the minimum amount of DCM, loaded onto a silica column, and eluted with a gradient of 10% EtOAc/hexanes to 39% EtOAc/hexanes to give 19.19 g of product by passage through 1H Purity as determined by NMR was approximately 95%