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However, the mechanism of action of GIP in this paradox and the extent of its role in these outcomes remains unclear

Western blot analysis Cells or purified microvesicles were lysed on ice in RIPA buffer (50 mM Tris (pH 7.4), 150 mM NaCl, 0.1% SDS, 5 mM EDTA, 2 mM sodium pyrophosphate, 25 mM -glycerophosphate, 1% Triton X-100, 10 mM NaF, 0.5 mM DTT, 1 mM PMSF, Protease Inhibitor Cocktail), Supernatants containing the solubilized proteins were quantified by BCA Protein Assay Kit

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