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The concentrations of protein extracts were determined by using the BCA Protein Assay Kit (Thermo Fisher Cat #23225), then protein samples were mixed with 3 loading buffer and loaded in equal amounts into the wells of 412% Bis-Tris protein gels (Bio-Rad) to transfer the proteins to PVDF membranes (Millipore)

Hypotension and Orthostatic Changes Dehydration from gastrointestinal side effects and reduced fluid intake (due to decreased appetite) can lead to volume depletion and low blood pressure

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If a patient, after consulting with a prescriber, has a specific medical need that cannot be met with an FDA-approved medication, then patients can be prescribed patient-specific compounded medications that may include, if appropriate, two or more active ingredients that would be impractical or impossible to administer with two separate prescription orders for each medication to fit the patients unique needs

The medications work by slowing down movement of food through the stomach and curbing appetite, thereby causing weight loss