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Subsequently, the cells were fixed with 4% paraformaldehyde and permeabilized with PBS containing 0.1% Triton X-100 (BL934B, Baisha, Anhui, China) to facilitate the introduction and hybridization of Cy3-labeled oligo-adenosine (dT) probe (26-4320-02, Genelink, USA), which specifically binds to the Poly-A tail of mRNA

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