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In step-a), H-Gln(Trt)-AIa-AIa-Lys(C18diacidmono-t-butyI-y-Glu(AEEA-AEEA)-OtBu)-Glu (OtBu)-Phe-IIe-AIa-Trp(Boc)-Leu-VaI-Arg(pbf)-Gly-Arg(pbf)-Gly-OtBu (fragment-6) was condensed with Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-OH (fragment-3) in presence of coupling agent obtain Fmoc-Ser(tBu)-Tyr(tBu)-Leu-Glu(OtBu)-Gly-Gln(Trt)-AIa-AIa-Lys(C18 diacidmono-t-butyI-Y-Glu(AEEA-AEEA)-OtBu)-Glu(OtBu)-Phe-IIe-AIa-Trp(Boc)-Leu-VaI- Arg(pbf)-Gly-Arg(pbf)-Gly-OtBu in in-situ manner

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