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Equal amounts of protein (20 g per lane) were loaded onto 10% SDS-PAGE gels, transferred to PVDF membranes (Bio-Rad), and blocked with 5% non-fat milk in TBS-T buffer

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Protein samples were mixed with Laemmli sample buffer, boiled at 95C for 5 minutes, and separated on a 12% SDS-polyacrylamide gel

This study investigated the role of ferroptosis in T

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