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Quarta C, Clemmensen C, Zhu Z, et al

The resulting species were compared with a reference control solution containing only AX kept under the same conditions, following two methodologies: (i) after incubation in PBS in inert atmosphere, mixtures were lyophilized and the resulting solids were dissolved in D 2 O and analyzed by 1 H-NMR

Once internal standard concentrations were obtained, the peak areas of the unlabelled metabolites in the samples of culture medium were compared with the peak area of the quantified internal standard to determine the metabolite concentration in the sample