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All these properties make GLP-1 a potent anti-diabetic agent

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For BrdU staining, the primary BrdU antibody was added together with 0.5 U l 1 DNAse (Sigma, D5025) and 1 mM MgCl 2 (Sigma, M8266) in the blocking solution and incubated for 30 min at room temperature

When to extend time at a dose level The phase 2 trial protocols allowed participants to remain at their current dose for an extra two to four weeks before increasing if they experienced significant nausea

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