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A microplate reader (Synergy Mx, Biotek Instruments Inc., Winooski, VT, USA) were used for determination of all activities and scaled down for semi-high throughput to obtain linear time and protein concentration dependence

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Figures 6 and 7 indicate that CB2 receptor blockade did not reduce the secretion of NO from LPS-stimulated BV-2 cells in the absence of p-aminophenol and AM404

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