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The test peptides are solubilized in DMSO and diluted in reaction buffer containing 5 g of membrane in 20 mM HEPES pH 7.4, 50 mM NaCl, 5 mM MgCl 2 , 40 g/ml saponin, 0.1% BSA, and 500 pM 35 S-labeled GTPS for 30 minutes at room temperature