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Cardiac fibroblasts promote ferroptosis in atrial fibrillation by secreting Exo-miR-23a-3p targeting SLC7A11

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If clinical suspicion persists despite low or negative antibody levels, fine-needle aspiration biopsy (FNA) and cytologic examination are recommended

Insoluble immune complexes were rinsed once with Co-IP buffer then eluted by 0.1 mM citrate (pH3.0) twice for 5 min, resolved by sodium dodecyl sulfatepolyacrylamide gel, and transferred to nitrocellulose