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Paraffin sections with formalin or PLP fixation were used for immunohistochemistry for acrolein (acrolein-lysine adduct), perilipin 2, phosphorylated-histone H2AX (-H2AX), and high mobility group box protein 1 (HMGB1) as listed in Table 1
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Once this interaction is disrupted: p53 is released from the nucleus Cellular self-destruction pathways are activated Senescent cells undergo apoptosis This selective mechanism has made FOXO4-DRI one of the most studied senolytic peptides in modern aging research
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