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The blastocysts were initially washed 3 times in a 0.1% PBS-PVA solution, followed by fixation in a 3.7% paraformaldehyde solution (at room temperature for 30 min)

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Strategies to minimize reactions include rotating injection sites consistently to avoid repeated trauma to the same area, ensuring the solution is at room temperature before injection (cold injections can increase irritation), injecting slowly rather than quickly, and using a smaller gauge needle for less tissue trauma

Some people choose to take N-Acetyl Cysteine (NAC) to help their body produce more of its own glutathione