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The validation accuracy of the suggested model initially stood at 66% but subsequently rose to 98%

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The inflated pancreas was dissected, and islets were isolated using Histopaque-1119 and 1083 (Sigma-Aldrich) gradients and cultured at 37 C, 5% CO 2 in RPMI supplemented with 10% FBS (Gibco), 100 units per ml penicillin and 100 g ml 1 streptomycin