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For tumour xenograft models, 1 10 6 HCC-1806 cells or 5 10 6 PC3 cells were injected into the right fourth mammary fat pad or hind flank of athymic nude NU/J mice (The Jackson Laboratory, 002019) in 100 l sterile PBS or a 1:1 mixture of cell suspension in PBS and Matrigel (Corning, 356237) using a 29-gauge 1 ml insulin syringe

Synthetic RNA Oligonucleotides and Transfection We purchased miRNA-146a-3p mimics, miRNA-146a-3p inhibitors, and a non-sense sequence for use as a miRNA negative control (NC) from Genepharma (Shanghai, China) (Supplementary Table 2)

(Peptide Sciences) Use in post-treatment settings: e.g., after chemo/radiation when senescent cells accumulate and impair recovery, or in surgical recovery settings

Every subsequent draw from the same vial occurs from an increasingly contaminated solution

Bodybuilders and athletes use it for recovery support, not direct mass gain