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Briefly, 75 L of the diluted extract was combined with 25 mL of freshly prepared FRAP reagent and 225 L of distilled water

Of note, almost 15% of patients were lost to follow-up in both groups ( p = 0.7, data not shown)

Some patients report fewer symptoms when injecting in the evening, allowing overnight sleep to mask early nausea

The luciferin formed is detected in a coupled reaction using Ultra-Glo Recombinant Luciferase that generates a glow-type luminescence that is proportional to the amount of glutathione present in cells