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Candida albicans Sap6 amyloid regions function in cellular aggregation and zinc binding, and contribute to zinc acquisition

If the room was kept at a standard temperature and the vial was not in direct sunlight, it is likely still safe to use

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The narrow airway could also be due to excess body weight

Following incubation, membranes were washed three times with TBST (5 min each) and incubated for 2 h at room temperature with alkaline phosphatase (AP)-conjugated secondary antibodies: goat anti-mouse (ab97020, Abcam 1:5000 dil.) or goat anti-rabbit (#ab6722, Abcam 1:3000 dil.), depending on the host species of the primary antibodies