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Briefly, metabolites from 3 10 6 cells were extracted on ice with 0.8 ml pre-cold 80% methanol (80 C), and cell rupture was achieved by a Bullet Blender Storm 24 (Next Advance, Troy, NY) with metal beads, followed by five freeze-thaw out cycles

Activation of NRF2 reduces iron absorption, limits ROS production, and enhances cellular antioxidant capacity (87)

i) LASSS-treated HGF, at as low as 1 ng/ml that is below 2.510 ng/ml of the optimal final-concentration range in culture media (supplementary Figure 1a, left light-blue bar ), activates satellite cells to the maximum level presented by the positive control ( black bar PC , 5 ng/ml HGF)

However, in ME2 homozygously deleted PDAC cell lines, its isoenzyme ME3 plays the compensatory roles for intracellular NADPH homeostasis

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