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The amount of water you add determines the concentration and directly affects measurement accuracy

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On top of the already discussed benefits for energy levels and combating stress, taking vitamin B12 on an empty stomach will aid absorption by creating an acidic environment the same goes for all nutrients that are not fat-soluble

[13] Chloramphenicol has been shown to blunt reticulocyte response to cyanocobalamin, while ethanol, colchicine, and nitrous oxide interfere with cobalamin metabolism or utilization, each warranting clinician awareness when evaluating suboptimal treatment outcomes

ESPEN micronutrient guideline