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The CFSE-labeled cells were plated in 96-well U-bottom culture plates at a concentration of 3.0 10 5 cells per well in RPMI-1640 cell culture medium supplemented with 2 nM l-glutamine, 50 g/mL gentamicin (Life Technologies, Beverly, MA, United States), 10% human AB serum (Sigma), and MEM nonessential amino acids and 55 M -mercaptoethanol (Gibco), known as complete RPMI-1640 (cRPMI-1640) and rested overnight at 37C/5% CO 2

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